KS3 · Biology

Food tests for nutrients

Iodine turns ground-up potato blue/black, but meat stays brown/orange. That 'nothing happened' is an answer too.

Read the result

What does the colour tell you?

Each card is a food, the reagent it was tested with and the colour it ended up. Start with starch: which results say yes, which say no, and which can't tell you at all? Pick a card, then pick where it goes.

Iodine solution is the test for starch. It starts brown/orange.

Still to sort

Starch present (0)

Iodine turned blue/black.

No starch (0)

Iodine stayed brown/orange.

Where the line is: Staying brown/orange is not a failed test. Iodine stayed its starting colour, and that is the answer: no starch.

This test can't tell you (0)

The reagent used tests for something else.

Where the line is: 'Can't tell' is different from 'no'. A reagent that stayed its starting colour gives a 'no' for the food it tests for. It gives no answer at all about anything else.

7 of 7 still to sort.

Sorted them all? Switch to Question 2 and sort the same results by how much reducing sugar they show.

Two tests, side by side

Iodine testvsBenedict's test

Two tests that are easy to mix up. Read across each row.

Focus

Heated?

Iodine test

No. Add the iodine and look.

Benedict's test

Yes, in a water bath.

The insight

Only the Benedict's test is heated. Its colour needs time in the water bath to develop.

What it tests for

Iodine test

Starch

Benedict's test

Reducing sugars, such as glucose, or fructose found in fruit

Colour change

Iodine test

Starts brown/orange. Turns blue/black if starch is there.

Benedict's test

Starts blue. Changes colour if reducing sugar is there.

Where the test is done

Iodine test

White spotting tile

Benedict's test

Boiling tube

How much reagent

Iodine test

One or two drops

Benedict's test

An equal volume to the food suspension

Safety

Iodine test

Wear goggles. Iodine is an irritant.

Benedict's test

Wear goggles. Benedict's solution is an irritant.

Your turn

Fill in the Benedict's method

You want to find out whether a solid food contains reducing sugar. Some steps are missing. Choose each one.

  1. Put your goggles on, then grind the food into small pieces with a pestle and mortar.Goggles because Benedict's solution is an irritant.
  2. Add distilled water to make a suspension.Tiny bits of food float in the water but don't dissolve.
  3. missing step
Which line is step 3?

Put it in order

The Benedict's colour scale

How much reducing sugar each Benedict's colour shows

1 · No reducing sugar4 · A large amount
  1. Orange

  2. Blue

  3. Brick red

  4. Green/yellow

What does it really tell you?

Brick red. So what?

A food is tested with Benedict's solution in the water bath. It turns brick red.

Which is closest to what you think this result tells you?
How sure are you?

WHAT YOU'VE LEARNED

A quick recap of today's lesson.

What you need to know

  • A food test shows whether a specific type of food is present in a sample. A reagent is the chemical used for the test: it changes colour to show whether the food substance is there.
  • Reagents can test for large food molecules, including protein, fat and carbohydrates such as starch. They can also test for smaller molecules, such as the sugars made when starch is broken down.
  • Starch is a large, complex carbohydrate. The body has to break it down into much smaller molecules, called sugars, before it can be absorbed.
  • Iodine test for starch: iodine solution is brown/orange and turns blue/black if starch is present. If it stays brown/orange, there is no starch.
  • Benedict's test for reducing sugars (such as glucose, or fructose found in fruit): Benedict's solution is blue. After heating, it stays blue (none) or turns green/yellow (traces), orange (moderate) or brick red (large amount).
  • Both tests are qualitative: they show whether a substance is present, or an approximate range. A quantitative test gives a measurable number.

The big picture

A food test uses a reagent, a chemical that changes colour to show whether a type of food is present. Iodine tests for starch: brown/orange turns blue/black if starch is there. Benedict's tests for reducing sugars: after a water bath, blue turns green/yellow, orange or brick red as the amount rises. A reagent that stays its starting colour means 'not present'. Both tests are qualitative: presence or a rough range, never an exact figure.

Key points

1Read the reagent before the colour. Iodine only answers 'starch or not?' and Benedict's only answers 'how much reducing sugar?'.
2A reagent that stays its starting colour hasn't failed. Iodine staying brown/orange means no starch, and Benedict's staying blue means no reducing sugar.
3Only the Benedict's test is heated: a boiling tube in a water bath at about 60 °C for about five minutes. The iodine test uses a white spotting tile and no heat.
4Bread and potato turn iodine blue/black, so they contain starch. Meat leaves it brown/orange. Glucose turns Benedict's brick red, and milk leaves it blue.
5Wear goggles for both tests, because iodine and Benedict's solution are both irritants.

Worked example

Problem

A student has a mystery food and wants to answer two questions: does it contain starch, and does it contain reducing sugar? The iodine result stays brown/orange. The Benedict's result, after the water bath, is green/yellow. What can the student conclude, and what can't they?

⚠ Watch out

Mixing up the two methods: heating the iodine test, or doing the Benedict's test on a spotting tile. Only the Benedict's test goes in a boiling tube in a water bath. And don't read 'no colour change' as a failed test. It means the food substance isn't there.

🧠

Memory hook

Only Benedict's gets the hot bath, and its colours go from 'cold' to 'hot': blue (none), green/yellow (traces), orange (moderate), brick red (lots). Iodine stays cool. Brown/orange means no starch, and blue/black means starch is there.

✓

Check yourself

Cover the page. Name both reagents, the colour each starts and the colour that means 'yes'. Which test needs a water bath? Why can't brick red give an exact amount?

Flashcards

(14)
What does a food test show?
Whether a specific type of food is present in a sample.
What is a reagent?
A chemical used to test for a type of food. It changes colour to show whether the food substance is there.
Which reagent tests for starch?
Iodine solution.
Iodine solution: starting colour, and colour if starch is present?
Starts brown/orange. Turns blue/black if starch is present.
Iodine stays brown/orange. What does that tell you?
There is no starch. Staying the starting colour is a result, not a failed test.
Which reagent tests for reducing sugars, and what colour is it to start with?
Benedict's solution. It is normally blue.
Give two examples of reducing sugars.
Glucose, and fructose (found in fruit).
What does each Benedict's colour show?
Blue: none. Green/yellow: traces. Orange: moderate amount. Brick red: large amount of reducing sugar.
Which step is only in the Benedict's test?
Heating the boiling tube in a water bath at about 60 °C for about five minutes, so the colour can develop.
What is a suspension?
A mixture where tiny food particles float in the water but do not dissolve.
Why wear goggles for both food tests?
Iodine and Benedict's solution are both irritants.
Qualitative or quantitative: what's the difference?
A qualitative test shows whether something is present, or a rough range. A quantitative test gives a measurable number.
Why does the body break starch down?
Starch is a large, complex carbohydrate. It must be broken into much smaller sugars before it can be absorbed.
Iodine test on bread, potato and meat: what happens?
Bread and potato turn it blue/black (starch present). Meat leaves it brown/orange (no starch).

Tap any card to flip it, or use Study as deck to go through them one at a time. In the full lesson these run as a spaced-repetition deck — you rate each card Hard, Good or Easy and the tricky ones keep coming back until they stick.

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How this lesson was checked. This KS3 Biologylesson was published through Lightbulb Learning's human-designed editorial process — the educational standards, accuracy rules and publication checks it must pass were authored and approved by Philip Halpin. It passed subject-specific assessment, automated educational checks and technical publication verification before going live (publication checks completed 29 September 2026). Published pages are monitored, human spot-checking is ongoing across the lesson library, and anything found wrong is corrected or withdrawn. How our lessons are made and checked. Spotted a mistake? Email hello@lightbulblearning.co and we'll review it.